![]() ![]() In contrast there was moderate to strong expression of IFN-γ in five out of six T-cell lines in atopic keratoconjunctivitis. IL-4 was only detected at the mRNA level and was expressed in four out of five T-cell lines in the vernal keratoconjunctivitis group. ![]() Conjunctival T-cell lines from atopic keratoconjunctivitis produced selective increases in IFN-γ, IL-10 and IL-13 ( P < 0.01), those from vernal keratoconjunctivitis produced increased IL-5 ( P < 0.01) whereas T-cell lines from giant papillary conjunctivitis produced only low levels of cytokines. ![]() All were HLA-DR + (> 80%) with some HLA-DQ expression. Resultsįollowing four to six rounds of stimulation, the conjunctival T-cell populations were CD3 + (> 93%), with variable levels of CD4 and CD8 expression. Cytokine production was quantified by immunoassays and more sensitive molecular techniques were used to investigate cytokine mRNA expression to identify the presence of interleukin (IL) -2, IL-4 and interferon (IFN) -γ transcripts. The lines were immunophenotyped by flow cytometry. T cells were isolated from conjunctival biopsies and non-specifically expanded into lines. The objective was to compare the cytokine profiles of conjunctival T-cell lines from patients with different forms of chronic allergic eye disease. Interactions between these cells are thought to play an important immunopathogenic role in these disorders (giant papillary conjunctivitis vernal keratoconjunctivitis atopic keratoconjunctivitis). Activated CD4 + T cells, mast cells and eosinophils are the main cytokine-producing cell-types infiltrating the conjunctiva during chronic allergic eye diseases. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |